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dylight 649 conjugated streptavidin  (Vector Laboratories)


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    Structured Review

    Vector Laboratories dylight 649 conjugated streptavidin
    Dylight 649 Conjugated Streptavidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dylight+649+conjugated+streptavidin/DyLight+649+Streptavidin/pmc11708056-230-9-13
    Average 93 stars, based on 66 article reviews
    dylight 649 conjugated streptavidin - by Bioz Stars, 2026-09
    93/100 stars

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    other:

    Article Title: Dendritic cell-specific transmembrane protein is required for synovitis and bone resorption in inflammatory arthritis
    Article Snippet: We detected primary antibodies with DyLight 649-conjugated streptavidin (SA-5649-1, Vector laboratories, Burlingame., CA), and Cy3-conjugated donkey anti-goat IgG (705-166-147, Jackson ImmunoResearch Laboratories, West Grove, PA) or AF647 donkey anti-rabbit antibodies (111-606-152, Jackson ImmunoResearch Laboratories, West Grove, PA) for 1-hour at room temperature.

    Article Title: Dendritic cell-specific transmembrane protein is required for synovitis and bone resorption in inflammatory arthritis.
    Article Snippet: We detected primary antibodies with DyLight 649- conjugated streptavidin (SA-5649-1, Vector laboratories, Burlingame., CA), and Cy3-conjugated donkey anti-goat IgG (705-166-147, Jackson ImmunoResearch Laboratories, West Grove, PA) or AF647 donkey anti-rabbit antibodies (111-606- 152, Jackson ImmunoResearch Laboratories, West Grove, PA) for 1-hour at room temperature.

    Incubation:

    Article Title: Pericyte-specific expression of PDGF beta receptor in mouse models with normal and deficient PDGF beta receptor signaling
    Article Snippet: Following serial SSC washes, sections were rinsed with PBS were incubated with biotin-conjugated Lycopersicon esculentum lectin (Vector Laboratories) overnight at 4°C. .. To visualize brain microvascular endothelial cells and PDGFRβ, sections were incubated with Dylight 649-conjugated streptavidin (Vector Laboratories) and Alexa Fluor 488-conguated donkey anti-goat IgG (Invitrogen), respectively, for 1 hour at room temperature. .. All images were acquired using a custom-built Zeiss LSM 510 meta confocal laser scanning microscope with a Zeiss LD LCI Plan-Apochromat 25×/0.8 Imm Korr DIC, C-Apochromat 40X or 63X water immersion objective (Carl Zeiss Microimaging Inc.; Thronwood, NY).

    Article Title: In vivo monitoring of active subretinal fibrosis in mice using collagen hybridizing peptides.
    Article Snippet: To confirm binding specificity of targeted versus control sCy7.5-CHPs, R-CHPs (2 μM concentration; 3Helix) together with sCy7.5-CHPs (either targeted or control; 3Helix) were added with the primary antibody in 1× PBS without heat-mediated pretreatment. .. The next day, the flat mounts were washed five times for 5 min with 1× PBS and then incubated for 2 h at room temperature with DyLight-488-conjugated (1:100, #SA-5488; Vector Laboratories) or DyLight-649-conjugated streptavidin (1:100, #SA-5488; Vector Laboratories) and donkey anti-rabbit immunoglobulin G (IgG) (1:200, Alexa Fluor 647, #A31573; Life Technologies) secondary antibody in 1× PBS. .. RPE/choroid tissue was then washed five times for 5 min with 1× PBS at room temperature before being mounted on Superfrost glass slides using fluorescent mounting medium (#S3032; Dako).

    Article Title: Identification of SLC35A1 as an essential host factor for the transduction of multi-serotype recombinant adeno-associated virus (AAV) vectors.
    Article Snippet: We conducted a genome-wide CRISPR/Cas9 screen in suspension 293 F cells transduced with rAAV5.. The highly selected genes revealed after two rounds of screening included the previously reported KIAA0319L, TM9SF2, and RNF121, along with a cluster of genes involved in glycan biogenesis, Golgi apparatus localization, and endoplasmic reticulum penetration.. In this report, we focused on solute carrier family 35 member A1 (SLC35A1), a Golgi apparatus-localized cytidine 5’-monophosphate-sialic acid (CMP-SIA) transporter.

    Article Title: In vivo monitoring of active subretinal fibrosis in mice using collagen hybridizing peptides
    Article Snippet: To confirm binding specificity of targeted versus control sCy7.5-CHPs, R-CHPs (2 μM concentration; 3Helix) together with sCy7.5-CHPs (either targeted or control; 3Helix) were added with the primary antibody in 1× PBS without heat-mediated pretreatment. .. The next day, the flat mounts were washed five times for 5 min with 1× PBS and then incubated for 2 h at room temperature with DyLight-488-conjugated (1:100, #SA-5488; Vector Laboratories) or DyLight-649-conjugated streptavidin (1:100, #SA-5488; Vector Laboratories) and donkey anti-rabbit immunoglobulin G (IgG) (1:200, Alexa Fluor 647, #A31573; Life Technologies) secondary antibody in 1× PBS. .. RPE/choroid tissue was then washed five times for 5 min with 1× PBS at room temperature before being mounted on Superfrost glass slides using fluorescent mounting medium (#S3032; Dako).

    Article Title: Identification of SLC35A1 as an essential host factor for the transduction of multi-serotype recombinant adeno-associated virus (AAV) vectors
    Article Snippet: Cells were fixed with 4% paraformaldehyde and blocked with carbo-free blocking solution (#SP-5040-125, Vector Laboratories). .. Cells were then incubated with biotinylated lectins followed by DyLight 649-conjugated streptavidin (#SA-5649-1, Vector Laboratories). ..

    Article Title: Identification of SLC35A1 as an essential host factor for the transduction of multi-serotype recombinant adeno-associated virus (AAV) vectors
    Article Snippet: Cells were fixed with 4% paraformaldehyde and blocked with a carbo-free blocking solution (#SP-5040-125, Vector Laboratories). .. Cells were then incubated with biotinylated lectins followed by DyLight 649-conjugated streptavidin (#SA-5649-1, Vector Laboratories). ..



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